Frequently Asked Questions

Frequently Asked Questions

Listed below are some of the frequently asked questions regarding our Custom Services. Please Contact Antibody Solutions Customer Services for general program information and Technical Services for technical questions regarding your project:

Which program should I use?

f you have an existing hybridoma and need antibody produced from it, you should use MAbExpress. For development of new antibodies, use either Polyclonal Solutions or Monoclonal Solutions. The table below gives recommendations for each program. These are general guidelines and the "best" program will depend on your unique antigen, application, and goals.

Application	Monoclonal Solutions	Polyclonal Solutions
ELISA	++	+(paired with McAb)
Flow Cytometry	++	-
Western Blot	+/- (Ab dependent)	++
Immunoprecipitation	++ (better specificity)	++
Immunohistochemistry	+	++(if Ag-affinity purif.)
Neutralization	++	++
In Vivo Studies	++	+/-

(++) best to use; (+) usually OK; (+/-) may work; (-) not recommended

In addition, Monoclonal Solutions is recommended when long-term stability, reliability, and use of an antibody are required. More information can also be found under Antigen Selection in Tech Solutions.

How much antigen will I need and of what purity?

Where applicable, you will find the quantity of antigen required in the tables found on our custom services program information sheets for MAbExpress, Monoclonal Solutions, and Polyclonal Solutions. For peptide antigens, we can create peptide immunogens of sufficient quantity and purity (see PolyExpress with Peptide for Polyclonal Solutions). As for purity, the highest reasonably possible is recommended and should be at least 85%. Where high purity is difficult to accomplish, a compromise strategy is to immunize with antigen of lower purity and screen with antigen of higher purity.

What kind of guarantee can you give me?

The people of Antibody Solutions will always make their best efforts to obtain the desired results. We utilize appropriately maintained facilities, properly trained personnel, and established protocols. Any protocol that fails our internal controls or performance specifications is repeated at no additional charge to our customer. Our customers have told us:

"MAbExpress has reduced the delivery time in half compared to production by other companies using ascites." JM.

"Sera from all five of these rabbits was unsuccessfully affinity purified by (name withheld). All of your affinity purification’s from these rabbits worked....they (the name withheld company) do not check things the way you do." LK.

"Your PolyExpress Sera worked better in western blotting than the serum we got from (a well-regarded government research institution)."JJ.