More Polyclonal Solutions™ 

Additional Services

Affinity Purification

Obtain antigen-affinity purified antibody for the highest specificity. Ideal for immunohistological staining.

Protocol
Ag Req
(mg)
Sera purif (ml)
Delivery
(weeks)
Cat #
Price
(US$)
Peptide affinity*
10
100
2
CS
9610
995
Protein affinity*
25
i00
2
CS
9620
1195
Hapten affinity*
10
100
2
CS
9630
1395
Ea. additional purification
included
100
1
CS
9640
595
*Includes preparation of affinity matrix using appropriate customer supplied antigen. All include protein electrophoretic analysis for purity.
Immunoglobulin-G Purification

Obtain the Ig- fraction free of other serum proteins. A mixture of specific and non-specific antibodies.

Protocol
Ag Req
(mg)
Sera purif (ml)
Delivery
(weeks)
Cat #
Price
(US$)
Rabbit IgG
none
20
1
CS
9650
695
Goat IgG
none
10
1
CS
9660
695
Rat IgG
none
5
1
CS
9670
695
Mouse IgG
none
5
1
CS
9680
695
All include SDS-PAGE analysis for purity.

Analytical Procedures

To characterize the functional activity of your polyclonal antibody.

Protocol
Ag Req
(mg)
Ab Req*
(ml)
Delivery
(weeks)
Cat #
Price
(US$)
ELISA Ab titer
0.05
0.025 ml
2
CS
3110
495
ELISA Ag titer
0.05
0.025 ml
2
CS
3111
495
ELISA Ab
and
Ag titer
0.05
0.025 ml
2
CS
3112
695

*Sera or purified antibody at >0.1 mg/ml. Inquire regarding other analysis of antibody function.


Conjugations

Obtain custom conjugates of peptides and from purified antibody

Protocol
Ag Req
(mg)
Ab
(mg)
Delivery
(weeks)
Cat #
Price
(US$)
Peptide-KLH*
2mg
3
none
1
CS
1110
495
Biotin-Ab
none
up to 5
1
CS
1210
595
Fluorescein-Ab
none
up to 5
1
CS
1220
695
R-phyco-erythrin -Ab
none
up to 10
1
CS
1230
1195
Horseradish peroxidase-Ab
none
up to 5
1
CS
1240
695
*For customer supplied peptide. Antibody purification not included (see Purifications). Inquire regarding other conjugations or larger quantities

Polyclonal Antibody Characteristics

Serum Quality Comparison

Comparison of sera obtained from an anti-peptide antibody program from 3 sources. Sera obtained from a production bleed of a rabbit on day 60-70 of the protocol .

Source
Appearance
Quantity of sera, (ml)
IgG*
(mg/ml)
Antibody Solutions
Amber-Clear
25
8.2
Competitor 1
Brown-Hemolyzed
15
3.2
Competitor 2
Brown-Precipitate
16
5.6

*Amount of IgG obtained after purification by Protein A sepharose chromatography.

Variation in Antibody Responses

Antibody responses will vary. PolyExpress is designed to obtain usable antisera quickly from both "strong" (A) and "weak" (B) antigens. PolyMax is designed to obtain antibody of higher affinity and concentration for more demanding applications.

Animal Welfare

Our protocols are designed to minimize pain and discomfort to animals and to avoid duplication of procedures.
Animals are maintained in USDA and NIH licensed facilities.

Antibody Solutions
Tel: 888-843-1069 Fax: 650-938-4390 E-mail:


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