Cellestive: Flexible Pathways Toward Antibody Discovery

Our B-cell fusion and hybridoma generation platform immortalizes the immune response by combining optimized fusion techniques and proprietary growth medium formulations to promote diversity and stability of the hybridoma population. Single- cell sorting of true clones by Flow Cytometry both improves the fidelity of screening and expedites the discovery process, since as no further sub-cloning is required.

Hybridoma Libraries we produce on your behalf are designed to deliver enduring value: they’re cryopreserved to maximize efficiency and flexibility while also minimizing your overhead costs of future research. Our high- yield hybridoma culture system allows for multiplexed high-throughput screening, including binding and even functional assays to interrogate thousands of clones and identify desired hits in a single round of screening.

Direct interrogation and sequencing of single B-cells is a critical component of our antibody discovery platform, especially as the industry focus has continued to shift its focus to more difficult therapeutic targets. 

Robust single-cell isolation, screening and sorting capabilities are combined with optimized V(D)J amplification for multiple species and next- generation sequencing technology to yield antigen-specific B-cell sequences that maintain cognate VH and VL pairing.

In addition, De Novo gene synthesis and recombinant expression of antibody sequences in mammalian cells allows for rapid validation and lead selection.

A truly comprehensive antibody discovery platform requires the integration of multiple technologies and workflows to deeply interrogate the immune response while also delivering multiparameter functional screening data. 

Our Guided NGS approach combines single B-cell sequence informatics with the high-throughput functional screening capabilities of the Hybridoma Library platform to provide a road map for successful navigation of the immune repertoire. The end result is a more efficient and cost-effective platform for deep mining of the antigen-specific B-cell population.