1 min read
Development of a Defined Cloning and Growth Factor Formulation for Murine Hybridomas
By Antibody Solutions Research Team on Jan 5, 2021 12:50:00 PM
Conditioned media from cultured cells have long been used to increase the cloning efficiency, growth rate and antibody secretion of hybridomas. The potential sources of conditioned media include cultured splenocytes, peritoneal macrophages, and macrophage, endothelial or fibroblast cell lines. The conditioned media from these cultures contain a variety of cytokines that act as growth factors for hybridomas. However, the constituents of conditioned media are largely undefined. Conditioned media may contain cell metabolites, cellular contaminants, and other undefined components that may interfere with the bio-analysis of hybridoma-derived antibodies.
Topics: Posters Hybridoma Conditioned Media
Filter by Keyword
- Posters (21)
- Publications (15)
- Therapeutic Monoclonal Antibodies (4)
- Monoclonal (3)
- Multi-meric Membrane (3)
- Multi-pass transmembrane (3)
- Transgenic Animals (3)
- ELISAs (2)
- Human Therapeutic Antibodies (2)
- Hybridoma (2)
- SARS-CoV-2 (2)
- APOBEC3G (1)
- ARMER (1)
- ATX-GX (1)
- Alloy Therapeutics (1)
- Antibody Discovery (1)
- Antibody Generation (1)
- BRCA2 (1)
- CEM15 (1)
- Cadherin-11 (1)
- Carcinogenesis (1)
- Conditioned Media (1)
- Critical Reagents (1)
- Cystine Knot Peptides (1)
- D Protein (1)
- DLL4 (1)
- Fully Human (1)
- HIV-1 (1)
- HSA (1)
- IL-1 alpha (1)
- Immune B-cells (1)
- Immunization (1)
- Knottins (1)
- L-amino acids (1)
- L-selectin (1)
- LAM (1)
- LBAs (1)
- LOXL2 (1)
- Lymphangioleiomyomatosis (1)
- McAbs (1)
- OmniAb (1)
- OmniRat (1)
- PNAd (1)
- Pharmacokinetic (PK) (1)
- Prolactin (1)
- Secretion Capture Report Web (1)
- Stereochemistry (1)
- Therapeutic Targets (1)
- Tissue Culture (1)
- Transgenic H2L2 Mice (1)
- VEGF-A (1)
- VEGF-C (1)
- VEGF-D (1)
- p53 (1)