Unlocking the Full Potential of Rabbit Monoclonal Antibodies with Cellestive

Joshua Lowitz, Rick Chang, Emily Keshner, Megan Hawkins, Michael Trang & John S. Kenney

Introduction

Rabbits generate diverse, high affinity antibodies to a wide variety of antigens using the mechanism of gene conversion, which delivers an antibody repertoire different from mice and humans. However, rabbit monoclonal antibodies have traditionally been difficult to immortalize via hybridoma technology due to challenges such as instability and inefficient antibody secretion. Alternatives such as phage display to generate rabbit antibodies often result in the loss of natural pairing between heavy and light chains , while traditional single B-cell technologies can miss certain B-cell subsets and be cost prohibitive. 

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