0 min read
Science
Related posts

Discovery Platform for Therapeutic D-Proteins
Stereochemistry is a fundamental property of proteins that defines their shape and biologic function. Proteins made of L-amino acids have inherent limitations as drugs since they are degraded by proteases which reduce their halflife and are often neutralized by an immune reaction to the protein.

Chemical Synthesis and X-ray Structure of a Heterochiral {D-protein antagonist plus vascular endothelial growth factor} Protein Complex by Racemic Crystallography
Total chemical synthesis was used to prepare the mirror image (D-protein) form of the angiogenic protein vascular endothelial growth factor (VEGF-A). Phage display against D-VEGF-A was used to screen designed libraries based on a unique small protein scaffold in order to identify a high affinity ligand.

Therapeutic Targeting of Endothelial Ligands for L-selectin (PNAd) in a Sheep Model of Asthma
The homing of lymphocytes to peripheral lymph nodes is initiated by an adhesive interaction between L-selectin on lymphocytes and PNAd, a set of sialomucins that are constitutively displayed on high endothelial venules of lymph nodes.

Drosophila p53 is a Structural and Functional Homolog of the Tumor Suppressor p53
The importance of p53 in carcinogenesis stems from its central role in inducing cell cycle arrest or apoptosis in response to cellular stresses.

Regulation of BRCA2 Gene Expression by the SLUG Repressor Protein in Human Breast Cells
The expression of the breast cancer susceptibility protein BRCA2 is highly regulated in human breast, ovary, and pancreatic cells.

Production of Monoclonal Antibodies Using a Secretion Capture Report Web
We describe a novel method for the production of monoclonal antibodies using a secretion capture report web (SCRW). Following HAT selection in bulk culture, individual hybridomas are encapsulated in biotinylated agarose drops.