Antibody Sequencing

Making Confident Sequencing Decisions

Antibody Solutions eases the decision-making burden through sample preparation and our comprehensive toolbox of reagents for sequencing of mouse, rat, human, and transgenic immunoglobulin variable regions. We offer full-length antibody sequencing services, as well as bidirectional sequencing directly from Polymerase Chain Reaction (PCR) products. With Antibody Solutions at your side, you can acquire your antibody sequences rapidly, with high accuracy, and low barriers to entry.

Sequencing

Over the last couple of decades, the advantages of antibody fragment generation have become clear. They can be used as an antigen, a characterization tool, an antibody detection or delivery tool, and even as a manufacturing tool. Because FAb and F(Ab’)2 have so many applications, knowing when and where they’re best used is more important than ever.

Target Validation Through Sanger Sequencing

Many of our clients come to us with a clear target, or small number of targets, already in mind. Maybe they’re looking for targets in specific genomic regions, but need some help validating these targets. This is where Antibody Solutions can put Sanger sequencing to use. 

Sanger techniques, a tried-and-true process that combines the use of fluorophore-labeled dideoxynucleotides (ddNTPs) with regular deoxynucleotides (dNTPs), allow for super-accurate, narrowly focused target validation. Utilizing robust molecular biology techniques and Sanger methods, we help our clients achieve full-length, high-confidence reads of the entire antibody variable region. 

With years of technical knowledge and hands-on experience, we have successfully provided solutions for the full array of the mouse and rat models we commonly utilize, including all of the human antibody-producing transgenic rodent models.

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Cast a Wider Net with Next-Generation Sequencing

Next generation sequencing (NGS) has been quietly revolutionizing our industry for the last twenty years. While it is still an emerging technology, it has been used successfully to discover new, commercially available therapies.

At Antibody Solutions, our team has put the advantages of NGS techniques to work for our clients. Many past projects have been limited in scope due to the time and financial constraints that come with using Sanger sequencing to process large volumes of data. NGS affords our clients the chance to cast a wider net early in their campaign, allowing early and potentially unexpected discoveries to inform the direction of their discovery campaign.

Although many NGS techniques are relatively new to the market, Antibody Solutions works hard to ensure that they are accurate and reliable. Our expert personnel actively work to put clear and rigorous protocols in place, ensuring that faithful and actionable data is captured and provided to you, the customer. Because we’ve always tried to build a diverse and robust services platform, it was only natural that we would embrace NGS.

Service You Can Rely On – Regardless of Approach

Just as we have for the tens of thousands of antibody clones we’ve generated since 1995, we apply the science and technical know-how we’ve mastered to deliver your sequences with:

Speed

Speed

As in “two-to-four weeks” in most cases, thanks in part to highly efficient methods for mRNA purification, cDNA generation and targeted PCR to obtain high throughput of samples.

Accuracy

Accuracy

We generate full-length, bidirectional Sanger sequence data directly from PCR products using germline-tailored primers and avoid unnecessary additional steps that could introduce error.

Flexibility

Flexibility

In addition to conventional rodents, we are the only CRO to generate antibodies and their related sequences from all four of the non-exclusive human antibody-producing transgenic animal platforms available for therapeutic antibody discovery: Harbour Antibodies™ H2L2 mouse, Ligand’s OmniMouse® and OmniRat®, Trianni Mouse® and Alloy Therapeutics’ ATX-GK® mouse.

Decode Success Easier with Antibody Solutions

Whatever the target you have in mind – or the method you choose to get there – Antibody Solutions offers fast, reliable and high-quality sequencing services to advance your discovery along with a team of experts who can help you identify the best path forward. And whether your final goal is simply the sequence or recombinantly expressed purified antibody or molecular modeling of the CDRs, we have a solution that’s right for you.