Western Blotting Applications

Antibody Solutions has generated many monoclonal and polyclonal antibodies for Western Blotting applications along with optimized protocols to ensure accuracy and efficiency. For example, we have made a monoclonal antibody (AS55-P) to SLP-76, a cytoplasmic protein that is expressed in T lymphocytes and myeloid cells and is a substrate for ZAP70 protein tyrosine kinase. As shown in the below left image, our antibody detects SLP-76 in total lysate of T-cells but not in B-cell lysate. Activation of the T-cell receptor leads to ZAP70-mediated phosphorylation of SLP-76, which is shown by the right image by Western Blotting with commercial anti-phosphotyrosine antibody on SLP-76 immunoprecipitated from activated T-cells with AS55-P.