Isolating B cells can be a challenging exercise requiring careful planning, precise execution and the right technology. Over the years, the best method that we’ve found to help streamline and improve the efficiency and success of the process is Fluorescence-Activated Cell Sorting (FACS).
Surface immunoglobulin (SIg)-bearing B cells and antibody-secreting cells (ASCs), such as plasmablasts or plasmacytes, are two populations within the germinal center for isolating high-affinity, antigen-specific antibodies. Using the FACS method, SIg cells may be identified by Ag-staining of SIg, whereas ASCs require capture of antibody secreted by the cell prior to Ag-staining. In fact, the FACS method is one of the most efficient and successful methods to isolate B-cells from both surface Ig-bearing and Ig-secreting cells.
This webinar, hosted by Dr. John Kenney, our president and co-founder, and Josh Lowitz, our manager of project management, summarizes research we conducted in 2018 that explored the utility of a FACS-based approach for isolating B cells from either population.
You can access a recording and download a copy of the webinar here.
We hope you find this research useful and would welcome the opportunity to talk with your about how the FACS approach can help advance your next project. You can reach us via phone at (+1) 888.843.1069 or email.
Author of more than 40 publications, John’s current research interests include new technologies for improving therapeutic antibody discovery, properties of next-generation antibody-like molecules, and best practices for critical reagents used in biologics development.