Solutions

Monoclonal Antibody Discovery

Advanced Hybridoma Technology for Monoclonal Antibody Development

We are the leading CRO for Monoclonal Antibody Development using conventional mice and rats as well as human antibodies from transgenic animals.

Many features of our antibody development process make Antibody Solutions the obvious choice for your antibody development partner. For example, transgenic, hyperimmune, gene knockout, and human antibody transgenic mice or rats may be used with a wide variety of antigens. In addition, rapid (four-week) immunization protocols yielding high-affinity antibodies are available, and Hybridoma Libraries™ can be interrogated for antibody binding activity prior to cloning.

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Our process includes cloning of single-cells by flow cytometry that enables the analysis of true clones and does not require multiple rounds of sub-cloning. We also offer ELISA, FIA, flow cytometry, and functional assays of antibody activity as well as label-free affinity and epitope analysis.

Key benefits:

  • PEG fusion to enhance for Ab-secreting hybridomas
  • HAT Selection of the bulk culture eliminates unstable hybridomas
  • Our proprietary cloning/growth factor ensures outgrowth of productive hybridomas
  • A cryopreserved cell bank immortalizes the hybridoma population
  • Supernatant can be tested for antibody binding activity prior to cloning

Advantages of the Antibody Solutions platform:

  • Hyperimmune and gene knockout mice
  • Human Transgenic Animals
    • OmniAb
    • Harbour Antibodies
    • Trianni
  • Rapid (four-week) immunization protocols
  • Optimized hybridoma culture conditions
  • Single-cell flow cloning (no sub-cloning required)
  • High-throughput flow screening
  • High-affinity clones in as few as 10 weeks

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Shortened timeline with Hybridoma Library and flow cloning

Advantages over structural libraries:

  • Inducible system that can be driven to high sensitivity and specificity
  • In vivo affinity maturation in multiple species
  • Negative selection for self-Ag binding
  • Natural pairing of heavy and light chains
  • Ab secreted directly by the hybridoma, no cloning for expression
  • Mammalian post-translational modification of Ab
  • Fewer potential downstream issues