Single Cell RNA Sequencing (scRNA-Seq) identifies the amino acid sequence that determines the properties of your antibody. Once sequences are in hand, recombinant expression provides a flexible and ready means of antibody production. Sequencing and expression of your single B-cells and hybridomas allows you to concentrate your screening efforts on unique candidate sequences as opposed to function-first screening.
The perfect partner to any single B-cell discovery pathway
Antibody Solutions’ scRNA Sequencing and Expression procedures are designed for flexibility and utility, making them the perfect partner to any antibody discovery pathway. Regardless of which pathway (or pathways) you pursue, a sequence-first approach provides a full picture of your data from the antibody discovery work. It’s no hardship to dig deeper into your results and help you mine your data for a broader collection of candidates that conform to your target profile.
Integrated Into Our Workflow
Our scRNA Seq is completely integrated with our Activated, Memory, and Plasma B-cell pathways. Our in-house expression, coupled with functional analysis of the antibody, ensures a seamless process to identify antibodies and characterize leads.
Paired HL & Single Chain Repertoires
Obtain paired heavy and light chain sequencing of single B-cells or positive hybridomas. Solutions for single chain repertoire analysis are possible for VHH discovery. Our sequencing methods offer fidelity, flexibility and value.
Different Strains and Species? No Problem!
Antibody Solutions custom primers are designed to obtain sequences from different species, including conventional or transgenic strains, and ideal antibody isotype. Unique molecular identifiers (UMIs) overcome amplification‐associated biases, and obtain high-quality reads mapped to the appropriate reference genome.
It’s a Time-Saver
In as little as four weeks, obtain recombinantly expressed proteins in your desired format for testing and characterization. This saves you valuable time and resources, avoiding potential artifacts and false-positive clones that can arise from display-based expression methods.
Set yourself up for success
Single Cell RNA Sequencing and Expression is the perfect culmination of the Cellestive pathways, enabling the downstream development of your antibody leads. Whether that includes humanization, ADC construction, or the generation of bispecifics, our Single Cell RNA Sequencing and Expression program allows us to directly express your unique hits in a fit-for-purpose format.
And, our process enables the screening of greater quantities of candidates, at a faster rate, granting you better odds on identifying the most promising candidates.
Start Strong. And finish even stronger.
When you pursue single-cell RNA Sequencing and Expression through Cellestive, you can scan deeper into your candidate pool – and do it faster (which is no small advantage when time is on the line). Contact us today to discover how our Sequencing and Expression services can be the ideal capstone to your multipath B-cell discovery program.
